ACTIVITIES

Sri Sri Tissue Culture Lab

Sri Sri Tissue Culture Lab (Joint venture of SSCASR and SSIAST)

Vision of Poojya Gurudev

Preserve and propagate Ayurveda worldwide for healthy lifestyle and create a stress free society.

Promote and spread the science of Ayurveda to every nook and corner of this world. Provide value based quality education in Ayurveda through a blend of ancient wisdom and modern sciences.

Rejuvenate medicinal plant wealth for future generation through Plant Tissue Culture.

Plant Tissue Culture

Plant Tissue Culture is a technology evolved in proliferation of plants from living cell, tissues or organs of plants under aseptic conditions on a nutrient culture medium of known composition through unique plant potential called totipotency.

Micropropagation is mostly done by culturing shoot tips and it involves three stages: Initiation, Multiplication, Rooting and Hardening.

Advantages of Tissue Culture
  • The technique of tissue culture has the enhanced rate of multiplication within a short period of time and space and can be cultured through out the year irrespective of season. The plants produced through tissue culture are disease free, healthy and identical to parent types.
  • Smaller size of propagules are advantageous for storing and transporting as it takes lesser space.
  • Stocks of germplasm of endangered species can be maintained for many years using this method of propagation.
  • Shoot multiplication usually has a short cycle and each cycle results in logarithmic increase in the number of shoots.
  • Tissue culture method helps to maintain the genetic uniformity in the propagules and in growth stages as well. Tissue culture often produces more robust plants, leading to accelerated growth compared to similar plants produced by conventional methods like seeds or cuttings.
Research objective:
  • Identification of plants with high medicinal value under the guidance of faculty members of SSCASR.
  • Development and standardisation of protocol for tissue culture of medicinal plants.
  • Develop novel techniques for acclimatisation and maintenance of tissue cultured plants under the guidance of SSIAST.
  • Produce medicinal plant kit for domestic herbal garden.
  • Promotion of home gardening of Ayurvedic herbs to treat common ailments.
Lab set-up

Well established functional Plant Tissue Culture lab.

Congenial environment for tissue culture of plants with dedicated inoculation room and growth chamber Poly house facility for acclimatisation of tissue cultured plantlets.

Principle of Plant Tissue Culture

Totipotency:
Plant tissue culture relies on the fact that many plant cells have the ability to regenerate into a whole plant which is called totipotency.

Plasticity:
It is the ability of the plants to alter their metabolism, growth and development to best suit their environment.

Stages of Plant Tissue Culture:
  • Stage 1: Selection of mother plant and its maintenance.
  • Stage 2: Initiation and establishment of culture.
  • Stage 3: Multiplication of shoots.
  • Stage 4: Rooting of shoots.
  • Stage 5: Acclimatization or Hardening.
Stage 1: Selection of mother plant and its maintenance.

The herbal garden of Sri Sri College of Ayurvedic Science and Research Center and Hariyalee Seeds are the major source of disease free mother plant.

Stage 2: Initiation and establishment of culture.

A piece of plant tissue (explant) is cut from the plant, surface sterilized and placed on a sterile medium to achieve an aseptic culture. Appropriate growth regulators are added for the development of vegetative shoots.

Stage 3: Multiplication of shoots.

Combination of growth regulators are added to medium to produce replicates of plantlets, thus increasing their numbers considerably. They are able to give rise to new individual plant.

Stage 4: Rooting of shoots.

Growing shoots can be induced to produce adventitious roots by including specific growth regulators in the medium.

Stage 5: Acclimatization or Hardening.

Well grown, rooted shoot are removed from tissue culture and placed in soil and maintained in greenhouse for hardening. The acclimatized plants are later transferred to the field.